Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IgA antibody [EPR5367-76] (ab124716)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5367-76] to IgA
Suitable for: WB, IP, ELISA, IHC-P
Reacts with: Human

Product name

Anti-IgA antibody [EPR5367-76]
See all IgA primary antibodies
Description

Rabbit monoclonal [EPR5367-76] to IgA
Host species

Rabbit
Specificity

ab124716 reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. It does not react with other Ig.
Tested applications

Suitable for: WB, IP, ELISA, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Full length native protein (purified) corresponding to Human IgA.
Positive control
- Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5367-76
Isotype

IgG
Research areas

Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (ab124716)

Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma

All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 0.18 µg/ml (ab124716 at 1:1000 dilution (0.18µg/ml))

Lane 1 : Human plasma with NFDM/TBST
Lane 2 : Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma with NFDM/TBST

Blocking peptides at 5 % per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

Observed band size: 60 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)

ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)

ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-IgA antibody [EPR5367-76] (ab124716)

All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

Lane 1 : Human tonsil lysate
Lane 2 : Human plasma lysate
Lane 3 : Human spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa
Anti-IgA antibody [EPR5367-76] (ab124716)

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