Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IgA antibody [EPR5367-76] (ab124716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-76] to IgA
  • Suitable for: WB, IP, ELISA, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-IgA antibody [EPR5367-76]
    See all IgA primary antibodies

  • Description

    Rabbit monoclonal [EPR5367-76] to IgA

  • Host species

    Rabbit

  • Specificity

    ab124716 reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. It does not react with other Ig.

  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human IgA.

  • Positive control

    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5367-76

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (ab124716)
    Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (ab124716)

    Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma



    All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 0.18 µg/ml (ab124716 at 1:1000 dilution (0.18µg/ml))

    Lane 1 : Human plasma with NFDM/TBST
    Lane 2 : Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma with NFDM/TBST

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)

    ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)

    ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-IgA antibody [EPR5367-76] (ab124716)
    Western blot - Anti-IgA antibody [EPR5367-76] (ab124716)
    All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 37 kDa

  • Anti-IgA antibody [EPR5367-76] (ab124716)
    Anti-IgA antibody [EPR5367-76] (ab124716)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-IgA antibody [EPR5367-76] (ab124716)

© 2021 Astana Biomed Group LLP. All rights reserved.