Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)
![Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)](https://www.abcam.com/ps/products/270/ab270457/Images/ab270457-1-anti-bone-sialoprotein-antibody-epr2336750-immunohistochemistry-mouse-e145.jpg "Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23367-50] to Bone Sialoprotein
- Suitable for: IHC-P, IHC-Fr, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Bone Sialoprotein antibody [EPR23367-50]
See all Bone Sialoprotein primary antibodies -
Description
Rabbit monoclonal [EPR23367-50] to Bone Sialoprotein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseIP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse E14.5 tissue. IHC-Fr: Mouse E14.5 tissue. IP: Mouse fetal bone tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23367-50 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)
Immunohistochemical analysis of paraffin-embedded Mouse E14.5 tissue labeling Bone Sialoprotein with ab270457 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on bones of mouse E14.5 (PMID: 24927920). The section was incubated with ab270457 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)](https://www.abcam.com/ps/products/270/ab270457/Images/ab270457-3-anti-bone-sialoprotein-antibody-epr2336750-immunohistochemistry-frozen-mouse-e145-embryo.jpg)
Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-1-- permeabilized mouse E14.5 embryo tissue labeling Bone Sialoprotein with ab270457 at 1/500 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse embryonic bone. The nuclear counter stain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Secondary antibody is b150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)](https://www.abcam.com/ps/products/270/ab270457/Images/ab270457-2-anti-bone-sialoprotein-antibody-epr2336750-immunoprecipitation-mouse-fetal-bone-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)Bone Sialoprotein was immunoprecipitated from 0.35 mg Mouse fetal bone tissue lysate using ab270457 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse fetal bone tissue lysate 10ug
Lane 2: ab270457 IP in Mouse fetal bone tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270457 in Mouse fetal bone tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 sseconds.
The molecular weight observed is consistent with literature (PMID: 14647232).
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![Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)](https://www.abcam.com/ps/products/270/ab270457/Images/ab270457-4-benefits-of-recombinant-antibodies.png)
Anti-Bone Sialoprotein antibody [EPR23367-50] (ab270457)
