Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)
![Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)](https://www.abcam.com/ps/products/33/ab33912/Images/ab33912-450824-anti-mek4-mkk4-antibody-ep615y-westernblot-brain-rat.jpg "Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP615Y] to MEK4/MKK4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MEK4/MKK4 antibody [EP615Y]
See all MEK4/MKK4 primary antibodies -
Description
Rabbit monoclonal [EP615Y] to MEK4/MKK4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide within Human MEK4/MKK4 (C terminal). The exact sequence is proprietary.
Database link: P45985 -
Positive control
- WB: NIH/3T3 and HeLa whole cell lysates. Rat brain tissue lysate.
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General notes
This product was previously labelled as MEK4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab33912 is a purified IgG. -
Clonality
Monoclonal -
Clone number
EP615Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)Anti-MEK4/MKK4 antibody [EP615Y] (ab33912) at 1/1000 dilution (Purified) + Rat brain lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking buffer: 5% NFDM/TBST
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![Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)](https://www.abcam.com/ps/products/33/ab33912/Images/ab33912-450823-anti-mek4-mkk4-antibody-ep615y-westernblot-human-mouse.jpg)
Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)All lanes : Anti-MEK4/MKK4 antibody [EP615Y] (ab33912) at 1/4000 dilution (Purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking buffer: 5% NFDM/TBST
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![Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)](https://www.abcam.com/ps/products/33/ab33912/Images/ab33912-251573-anti-mek4-antibody-ep615y-western-blot.jpg)
Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK4 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab33912 observed at 44 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab33912 was shown to specifically react with MEK4 when MEK4 knockout samples were used. Wild-type and MEK4 knockout samples were subjected to SDS-PAGE. ab33912 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
