Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho T6, tri methyl K9)
- Suitable for: ChIP, WB, Dot blot, ICC/IF
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
Overview
-
Product name
Anti-Histone H3 (phospho T6, tri methyl K9) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho T6, tri methyl K9) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, Dot blot, ICC/IFmore details -
Species reactivity
Reacts with: Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (phospho T6, tri methyl K9).
Database link: Q71DI3 -
Positive control
- WB: Caenorhabditis elegans embryo lysate; HeLa histone preparation. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147) at 1/500 dilution + Caenorhabditis elegans embryo lysate at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Immunofluoescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (phospho T6, tri methyl K9) using ab272147 at a 1/50 dilution for 1 hour at room temperature (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue).
-
Western blot - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147) at 1/500 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
-
ChIP - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 2µg of ab272147 and 20µl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
-
Dot Blot - Anti-Histone H3 (phospho T6, tri methyl K9) antibody (ab272147)Dot Blot analysis of Histone H3 (phospho T6, tri methyl K9) using ab272147 at 1/1000 dilution.
Lane 1: T6.
Lane 2: pT6.
Lane 3: pT6 K9KMe1.
Lane 4: pT6 K9KMe2.
Lane 5: pT6 K9KMe3.
Lane 6: K9.
Lane 7: K9KMe1.
Lane 8: K9KMe2.
Lane 9: K9KMe3.
Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide.