Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17476(2)] to Histone H2A (acetyl K13)
Suitable for: PepArr, WB, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)]
See all Histone H2A primary antibodies
Description

Rabbit monoclonal [EPR17476(2)] to Histone H2A (acetyl K13)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse Human
PepArr	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and NIH/3T3 whole cell lysates untreated and treated with 500ng/ml TSA for 4 hours. ICC/IF: HeLa and NIH/3T3 cells untreated and treated with 500ng/ml TSA for 4 hours.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17476(2)
Isotype

IgG
Research areas

Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316)

All lanes : Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316) at 1/1000 dilution

Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 500ng/ml TSA for 4 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316)

All lanes : Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316) at 1/1000 dilution

Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 500ng/ml TSA for 4 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% BSA/TBST.
Peptide Array - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316)

ab177316 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with 500ng/ml TSA for 4 hours, labeling Histone H2A (acetyl K13) with ab177316 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The results show nuclear staining on TSA (500ng/ml for 4hr) treated HeLa cells.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^®594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:-

-ve control 1: ab177316 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^®594) (ab150120) secondary antibody at 1/1000 dilution.

-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells, untreated or treated with 500ng/ml TSA for 4 hours, labeling Histone H2A (acetyl K13) with ab177316 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The results show nuclear staining on TSA (500ng/ml for 4hr) treated NIH/3T3 cells.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:-

-ve control 1: ab177316 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.

-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291)at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) ab150077 secondary antibody at 1/1000 dilution.
Anti-Histone H2A (acetyl K13) antibody [EPR17476(2)] (ab177316)

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