Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13704] to Spata18 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Spata18 antibody [EPR13704] - BSA and Azide free
    See all Spata18 primary antibodies

  • Description

    Rabbit monoclonal [EPR13704] to Spata18 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250161 is the carrier-free version of ab180154. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250161 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13704

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)
    Western blot - Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)
    All lanes : Anti-Spata18 antibody [EPR13704] (ab180154) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : HT1080 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa



    This data was developed using ab180154, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)
    Immunocytochemistry - Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)
    This data was developed using ab180154, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Spata18 with ab180154 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)
    This data was developed using ab180154, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Spata18 with ab180154 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)
    Anti-Spata18 antibody [EPR13704] - BSA and Azide free (ab250161)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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