Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21084] to Vimentin (phospho S56) - BSA and Azide free
  • Suitable for: WB, Dot blot, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free
    See all Vimentin primary antibodies

  • Description

    Rabbit monoclonal [EPR21084] to Vimentin (phospho S56) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blot, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    Ab228854 is the carrier-free version of ab217673. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab228854 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)
    Flow Cytometry - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)

    Ab217673 staining Vimentin (phospho S56) in HeLa (human cervix adenocarcinoma epithelial cell) cells by Flow Cytometry. Cells were fixed using 80% Methanol and permeabilized with 0.1% Tween-20. The sample was incubated with primary antibody at 1:500 dilution. An Alexa Fluor® 488 Goat anti-rabbit IgG (ab150077) was used as a secondary antibody. Rabbit monoclonal IgG (ab172730) was used as an isotype control (left). Cells were pre-treated with 20 μg/ml RNase A for 30 minutes to eliminate the non-specific binding between RNA and PI (Propidium iodide). Vimentiin (phospho S56) is highly expressed in mitotic cells. (PMID: 16260496)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217673).

  • Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)
    Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)

    Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate 10 μg (Input).
    Lane 2: ab217673 IP in HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate.

    Exposure time: 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217673).

  • Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)
    Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)

    Dot blot analysis of Vimentin (phospho S56) labeled with ab217673 at 1/1000 dilution.

    Lane 1: Vimentin (phospho S56) peptide.
    Lane 2: Vimentin non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 minute.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217673).

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin (phospho S56) with ab217673 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing strong positive staining in HeLa cells in M phase.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217673).

  • Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)
    Anti-Vimentin (phospho S56) antibody [EPR21084] - BSA and Azide free (ab228854)

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