Anti-PAX6 antibody (ab238527)
Key features and details
- Rabbit polyclonal to PAX6
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PAX6 antibody
See all PAX6 primary antibodies -
Description
Rabbit polyclonal to PAX6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PAX6 aa 1-300. NP_000271.1
Database link: P26367 -
Positive control
- WB: SW480, 22Rv1, NCI-H460 and HepG2 cell lysates. Mouse brain, eye and rat brain tissue lysates; ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAX6 antibody (ab238527)All lanes : Anti-PAX6 antibody (ab238527) at 1/1000 dilution
Lane 1 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : 22Rv1 (Human prostate carcinoma cell line) cell lysate
Lane 3 : NCI-H460 (Human lung cancer cell line) cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse eye tissue lysate
Lane 7 : Rat brain tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 secondsBlocking buffer: 3% non-fat dry milk in TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab238527)Immunofluorescence staining of U-2 OS cells stained for PAX6 with ab238527 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
