Anti-Gli1 antibody (ab217326)
Key features and details
- Rabbit polyclonal to Gli1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Gli1 antibody
See all Gli1 primary antibodies -
Description
Rabbit polyclonal to Gli1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Gli1 aa 750 to the C-terminus (C terminal).
Database link: P08151 -
Positive control
- WB: Recombinant human Gli1 protein; U87, MCF7, HeLa, SKOV, HT1080, COLO320 and HepG2 whole cell lysate (ab7900). IHC-P: Human intestinal cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Gli1 antibody (ab217326)All lanes : Anti-Gli1 antibody (ab217326) at 0.5 µg/ml
Lane 1 : U87 whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : SKOV whole cell lysate
Lane 5 : HT1080 whole cell lysate
Lane 6 : COLO320 whole cell lysate
Lane 7 : HepG2 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 118 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)Immunohistochemical analysis of paraffin embedded mouse testis tissue staining Gli1 with ab217326 at 1µg/ml. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/100 dilution. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. DAPI was used as a nucelar counterstain (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)Immunohistochemical analysis of paraffin-embedded human intestinal cancer tissue labeling Gil1 with ab217326 at 1 µg/mL.
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Western blot - Anti-Gli1 antibody (ab217326)Anti-Gli1 antibody (ab217326) at 0.5 µg/ml + recombinant human GLI1 protein, 5 ng
Predicted band size: 118 kDa
