Anti-SP2 transcription factor antibody (ab229468)
Key features and details
- Rabbit polyclonal to SP2 transcription factor
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SP2 transcription factor antibody
See all SP2 transcription factor primary antibodies -
Description
Rabbit polyclonal to SP2 transcription factor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human SP2 transcription factor (internal sequence). The exact sequence is proprietary.
Database link: Q02086 -
Positive control
- WB: Jurkat, Raji, NCI-H929 whole cell lysates; A431 and HepG2 whole cell extracts. IP: Raji whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SP2 transcription factor antibody (ab229468)All lanes : Anti-SP2 transcription factor antibody (ab229468) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (Human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-SP2 transcription factor antibody (ab229468)All lanes : Anti-SP2 transcription factor antibody (ab229468) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-SP2 transcription factor antibody (ab229468)SP2 transcription factor was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 3 µg ab229468. Western blot was performed from the immunoprecipitate using ab229468. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Raji whole cell extract.
Lane 2: Control IgG IP in Raji whole cell extract.
Lane 3: ab229468 IP in Raji whole cell extract.
