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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Vimentin antibody (ab188499)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Vimentin antibody (ab188499)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Vimentin
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Vimentin antibody
    See all Vimentin primary antibodies
  • Description

    Rabbit polyclonal to Vimentin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Mammals, Cynomolgus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Vimentin aa 1 to the C-terminus. (Purified from E. coli).
    Database link: P08670

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa cell extract; HeLa, SH-SY5Y, HEK-293 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.07% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.

  • Western blot - Anti-Vimentin antibody (ab188499)
    Western blot - Anti-Vimentin antibody (ab188499)
    All lanes : Anti-Vimentin antibody (ab188499) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

    Predicted band size: 53 kDa

  • Western blot - Anti-Vimentin antibody (ab188499)
    Western blot - Anti-Vimentin antibody (ab188499)
    Anti-Vimentin antibody (ab188499) at 1/10000 dilution + HeLa cell extract

    Developed using the ECL technique.

    Predicted band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499)

    Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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