Anti-Vimentin antibody (ab188499)
Key features and details
- Rabbit polyclonal to Vimentin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Vimentin antibody
See all Vimentin primary antibodies -
Description
Rabbit polyclonal to Vimentin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Mammals, Cynomolgus monkey -
Immunogen
Recombinant full length protein corresponding to Human Vimentin aa 1 to the C-terminus. (Purified from E. coli).
Database link: P08670 -
Positive control
- WB: HeLa cell extract; HeLa, SH-SY5Y, HEK-293 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.
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All lanes : Anti-Vimentin antibody (ab188499) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Predicted band size: 53 kDa
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Anti-Vimentin antibody (ab188499) at 1/10000 dilution + HeLa cell extract
Developed using the ECL technique.
Predicted band size: 53 kDa
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Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.