Anti-SLUG antibody (ab106077)
Key features and details
- Rabbit polyclonal to SLUG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLUG antibody
See all SLUG primary antibodies -
Description
Rabbit polyclonal to SLUG -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human SLUG aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab133782) -
General notes
This antibody has only been tested on overexpressing cell lysates; we have no data on the detection of the endogenous protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).Target
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Function
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
Tissue specificity
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Omim: 602150 Human
- SwissProt: O43623 Human
- SwissProt: P97469 Mouse
- Unigene: 360174 Human
- Unigene: 4272 Mouse
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Alternative names
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
Images
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Western blot - Anti-SLUG antibody (ab106077)All lanes : Anti-SLUG antibody (ab106077) at 1 µg/ml
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 cell lysate overexpressing SNAIL protein
Lane 3 : MCF7 cell lysate transfected with vector control plasmid
Lane 4 : Untransfected MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes -
Western blot - Anti-SLUG antibody (ab106077)This image is courtesy of an anonymous AbreviewAll lanes : Anti-SLUG antibody (ab106077) at 1/2000 dilution
Lane 1 : MDA-MB-231 whole cell lysate
Lane 2 : MDA-MB-231 whole cell lysate - sh Slug 1
Lane 3 : MDA-MB-231 whole cell lysate - sh Slug 2
Lane 4 : MDA-MB-231 whole cell lysate - sh Snail 1
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 35,65 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 22°C.
Primary incubation at 4°C for 18 hours.
Protocols
Datasheets and documents
References (13)
ab106077 has been referenced in 13 publications.
- Yang Y et al. Tumor Suppressor microRNA-138 Suppresses Low-Grade Glioma Development and Metastasis via Regulating IGF2BP2. Onco Targets Ther 13:2247-2260 (2020). PubMed: 32214825
- Tian S et al. SERPINH1 regulates EMT and gastric cancer metastasis via the Wnt/ß-catenin signaling pathway. Aging (Albany NY) 12:3574-3593 (2020). PubMed: 32091407
- Sun P et al. circPCNX and Pecanex Promote Hepatocellular Carcinoma Cell Viability by Inhibiting miR-506. Cancer Manag Res 11:10957-10967 (2019). PubMed: 32099459
- He C et al. miR-10b suppresses cell invasion and metastasis through targeting HOXA3 regulated by FAK/YAP signaling pathway in clear-cell renal cell carcinoma. BMC Nephrol 20:127 (2019). PubMed: 30975094
- Chang Z Downregulation of SOX2 may be targeted by miR-590-5p and inhibits epithelial-to-mesenchymal transition in non-small-cell lung cancer. Exp Ther Med 18:1189-1195 (2019). WB ; Human . PubMed: 31316613
Images
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Western blot - Anti-SLUG antibody (ab106077)All lanes : Anti-SLUG antibody (ab106077) at 1 µg/ml
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 cell lysate overexpressing SNAIL protein
Lane 3 : MCF7 cell lysate transfected with vector control plasmid
Lane 4 : Untransfected MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
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Western blot - Anti-SLUG antibody (ab106077) This image is courtesy of an anonymous AbreviewAll lanes : Anti-SLUG antibody (ab106077) at 1/2000 dilution
Lane 1 : MDA-MB-231 whole cell lysate
Lane 2 : MDA-MB-231 whole cell lysate - sh Slug 1
Lane 3 : MDA-MB-231 whole cell lysate - sh Slug 2
Lane 4 : MDA-MB-231 whole cell lysate - sh Snail 1
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 35,65 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 22°C.
Primary incubation at 4°C for 18 hours.
