Anti-SLUG antibody (ab27568)
Key features and details
- Rabbit polyclonal to SLUG
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SLUG antibody
See all SLUG primary antibodies -
Description
Rabbit polyclonal to SLUG -
Host species
Rabbit -
Specificity
ab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates. -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig, Xenopus laevis
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Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (3) 1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).ICC Use at an assay dependent concentration.Notes WB
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).ICC
Use at an assay dependent concentration.Target
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Function
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
Tissue specificity
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 432368 Chicken
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 25554 Rat
- Entrez Gene: 379440 Xenopus laevis
- Omim: 602150 Human
- SwissProt: Q3MHQ4 Cow
- SwissProt: O43623 Human
see all -
Alternative names
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
Images
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Immunocytochemistry - Anti-SLUG antibody (ab27568)This image is courtesy of Don Newgreen and Dong Cheng Zhang, Murdoch Childrens Research InstituteTransverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
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Western blot - Anti-SLUG antibody (ab27568)All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaSecondary antibody - goat anti-rabbit HRP (preadsorbed)
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Western blot - Anti-SLUG antibody (ab27568)This image is courtesy of Archana Dhasarathy, Ph.D., NCAll lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 lysate overexpressing SNAIL protein
Lane 3 : MCF7 lysate tranfected with vector control plasmid
Secondary
All lanes : HRP-conjugated anti-rabbit antibody at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown). -
Immunocytochemistry - Anti-SLUG antibody (ab27568)This image is courtesy of an anonymous Abreviewab27568 staining mouse embryonic stem cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking with 0.1% BSA and 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (96)
ab27568 has been referenced in 96 publications.
- Sun X et al. Effect of Loureirin B on Crohn's disease rat model induced by TNBS via IL-6/STAT3/NF-?B signaling pathway. Chin Med 15:2 (2020). PubMed: 31911815
- Lai CC et al. Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32235811
- Bu H et al. Overexpression of PRC1 indicates a poor prognosis in ovarian cancer. Int J Oncol 56:685-696 (2020). PubMed: 31922238
- Meng Q et al. LINC00461/miR-4478/E2F1 feedback loop promotes non-small cell lung cancer cell proliferation and migration. Biosci Rep 40:N/A (2020). PubMed: 31934717
- Liu A et al. Relevance and prognostic ability of Twist, Slug and tumor spread through air spaces in lung adenocarcinoma. Cancer Med 9:1986-1998 (2020). PubMed: 31970942
Images
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Immunocytochemistry - Anti-SLUG antibody (ab27568) This image is courtesy of Don Newgreen and Dong Cheng Zhang, Murdoch Childrens Research InstituteTransverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
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Western blot - Anti-SLUG antibody (ab27568)All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaSecondary antibody - goat anti-rabbit HRP (preadsorbed)
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Western blot - Anti-SLUG antibody (ab27568) This image is courtesy of Archana Dhasarathy, Ph.D., NCAll lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 lysate overexpressing SNAIL protein
Lane 3 : MCF7 lysate tranfected with vector control plasmid
Secondary
All lanes : HRP-conjugated anti-rabbit antibody at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown). -
Immunocytochemistry - Anti-SLUG antibody (ab27568) This image is courtesy of an anonymous Abreview
ab27568 staining mouse embryonic stem cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking with 0.1% BSA and 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.
