Anti-Legumain antibody (ab232870)
Key features and details
- Rabbit polyclonal to Legumain
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Legumain antibody
See all Legumain primary antibodies -
Description
Rabbit polyclonal to Legumain -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Macaque monkey, Orangutan
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human Legumain aa 1 to the C-terminus. N-terminal tags. Expressed in E. coli. Full length mature chain including propeptide, not including signal peptide.
Database link: Q99538 -
Positive control
- WB: Recombinant human Legumain protein and rat placenta tissue. IHC-P: Human breast cancer, kidney, stomach, colorectal cancer, prostate and prostate cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Legumain antibody (ab232870)Anti-Legumain antibody (ab232870) at 1 µg/ml + Rat placenta tissue lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Legumain antibody (ab232870)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Legumain antibody (ab232870)Formalin-fixed, paraffin-embedded human kidney tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Legumain antibody (ab232870)Formalin-fixed, paraffin-embedded human stomach tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Legumain antibody (ab232870)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Legumain antibody (ab232870)Formalin-fixed, paraffin-embedded human prostate tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Legumain antibody (ab232870)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-Legumain antibody (ab232870)Anti-Legumain antibody (ab232870) at 1 µg/ml + Recombinant human Legumain protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDa
