Anti-Exonuclease 1 antibody (ab106303)
Key features and details
- Rabbit polyclonal to Exonuclease 1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Exonuclease 1 antibody
See all Exonuclease 1 primary antibodies -
Description
Rabbit polyclonal to Exonuclease 1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
-
Immunogen
Synthetic peptide corresponding to Human Exonuclease 1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129328) -
Positive control
- WB: Human bone marrow, testis and thymus tissue lysates and HeLa, HEK293, A549 and A431 whole cell lysates. ICC/IF: HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab106303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB 1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 94 kDa). Target
-
Function
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. -
Tissue specificity
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. -
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. -
Developmental stage
Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus. -
Post-translational
modificationsPhosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein. -
Cellular localization
Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase. - Information by UniProt
-
Database links
- Entrez Gene: 457856 Chimpanzee
- Entrez Gene: 9156 Human
- Entrez Gene: 305000 Rat
- Omim: 606063 Human
- SwissProt: Q9UQ84 Human
- Unigene: 498248 Human
-
Alternative names
- exo1 antibody
- EXO1_HUMAN antibody
- ExoI antibody
see all
Images
-
Western blot - Anti-Exonuclease 1 antibody (ab106303)All lanes : Anti-Exonuclease 1 antibody (ab106303) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 6 : Human testis tissue lysate - total protein (ab30257)
Lane 7 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The predicted molecular weight of Exonuclease 1 is 94 kDa (SwissProt), however we expect to observe a banding pattern around 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-Exonuclease 1 antibody (ab106303)All lanes : Anti-Exonuclease 1 antibody (ab106303) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 94 kDa
Exposure time: 20 minutes
The predicted molecular weight of Exonuclease 1 is 94 kDa (SwissProt), however we expect to observe a banding pattern around 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Exonuclease 1 antibody (ab106303)ICC/IF image of ab106303 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106303, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.
Protocols
Datasheets and documents
References (0)
ab106303 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-Exonuclease 1 antibody (ab106303)All lanes : Anti-Exonuclease 1 antibody (ab106303) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 6 : Human testis tissue lysate - total protein (ab30257)
Lane 7 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The predicted molecular weight of Exonuclease 1 is 94 kDa (SwissProt), however we expect to observe a banding pattern around 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-Exonuclease 1 antibody (ab106303)All lanes : Anti-Exonuclease 1 antibody (ab106303) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 94 kDa
Exposure time: 20 minutes
The predicted molecular weight of Exonuclease 1 is 94 kDa (SwissProt), however we expect to observe a banding pattern around 115 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Exonuclease 1 antibody (ab106303)ICC/IF image of ab106303 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106303, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.
