Human LDLR ELISA Kit (ab270212)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 55.8 pg/ml
- Range: 187.5 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LDLR ELISA Kit
See all LDL Receptor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.5% Inter-assay Sample n Mean SD CV% Serum 3 3.64% -
Sample type
Cell culture supernatant, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
55.8 pg/ml -
Range
187.5 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 98 97% - 100% Serum 96 93% - 100% Hep Plasma 87 83% - 90% EDTA Plasma 92 88% - 94% Cit plasma 102 96% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human LDLR ELISA Kit (ab270212) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LDLR protein in cell lysate, edta plasma, serum, urine, cit plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LDLR with 55.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Low-density lipoprotein receptor (LDLR) is well known receptor found clustered in clathrin-coated pits. LDLR mediates the endocytosis of low-density lipoprotein (LDL) and maintains the plasma level of LDL. Studies have shown LDLR as a primary method of entry for vesicular stomatitis virus in humans. Also reported other viruses such as hepatitis C entering the cells indirectly by association with LDL via LDLR-mediated endocytosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human LDLR Capture Antibody 1 x 600µl 10X Human LDLR Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human LDLR Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. -
Involvement in disease
Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). -
Sequence similarities
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
Post-translational
modificationsN- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
Cellular localization
Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments. - Information by UniProt
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Alternative names
- FH
- FHC
- LDL R
see all -
Database links
- Entrez Gene: 3949 Human
- Omim: 606945 Human
- SwissProt: P01130 Human
- Unigene: 213289 Human
- Unigene: 728190 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human LDLR standard curve in Sample Diluent NS. as described in Section 10.The LDLR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native LDLR in human serum and plasma samples.The concentrations of LDLR were measured in duplicates, interpolated from the LDLR standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 34.6 ng/mL in serum, 41.6 ng/mL in plasma (citrate) and 24.9 ng/mL in plasma (EDTA), and 31.4 ng/mL in plasma (heparin).
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Interpolated concentrations of native LDLR in human urine and cell culture supernatant samples.The concentrations of LDLR were measured in duplicates, interpolated from the LDLR standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 2% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 223.5 ng/mL in urine and 28.0 ng/mL in HepG2 cell culture supernatant.
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Interpolated concentrations of native LDLR in HepG2 cell extract based on a 200 µg/mL extract load.The concentrations of LDLR were measured in duplicate and interpolated from the LDLR standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 11.8 ng/mL in sample.
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Serum from nine individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 65 ng/mL with a range of 38 – 115 ng/mL.
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Sandwich ELISA - Human LDLR ELISA Kit (ab270212)To learn more about the advantages of recombinant antibodies see here.
