Anti-SGLT2 antibody (ab58298)
Key features and details
- Mouse monoclonal [3G8] to SGLT2
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
-
Product name
Anti-SGLT2 antibody [3G8]
See all SGLT2 primary antibodies -
Description
Mouse monoclonal [3G8] to SGLT2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment: GLFDKYLGAA TSLTVSEDPA VGNISSFCYR PRPDSYHLLR HPVTGDLPWP , corresponding to amino acids 228-278 of Human SGLT2
-
General notes
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
3G8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-SGLT2 antibody (ab58298)
Western blot against tagged recombinant protein immunogen using ab58298 SGLT2 antibody at 1ug/ml. Predicted band size of immunogen is 32 kDa
This image was generated using the ascites version of the product.
-
Flow Cytometry - Anti-SGLT2 antibody (ab58298)Overlay histogram showing HEK293 cells stained with ab58298 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58298, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
