Anti-ULBP1 antibody (ab238331)
Key features and details
- Rabbit polyclonal to ULBP1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ULBP1 antibody
See all ULBP1 primary antibodies -
Description
Rabbit polyclonal to ULBP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human ULBP1 aa 1 to the C-terminus. Full length chain without signal peptide, without propeptide.
Database link: Q9BZM6 -
Positive control
- IHC-P: Human testis and liver tissue. ICC/IF: HEK-293 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity 95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-ULBP1 antibody (ab238331)
HEK-293 (human epithelial cell line from embryonic kidney) cells stained for ULBP1 using ab238331 at a dilution of 1/33 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ULBP1 antibody (ab238331)Paraffin-embedded human testis tissue stained for ULBP1 with ab238331 at a 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ULBP1 antibody (ab238331)Paraffin-embedded human liver tissue stained for ULBP1 with ab238331 at a 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
