Anti-Galectin 3 antibody (ab227249)
Key features and details
- Rabbit polyclonal to Galectin 3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Galectin 3 antibody
See all Galectin 3 primary antibodies -
Description
Rabbit polyclonal to Galectin 3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human Galectin 3 (internal sequence). The exact sequence is proprietary.
Database link: P17931 -
Positive control
- WB: HeLa cell extract; A549, H1299, HCT 116 and MCF7 whole cell lysates; Galectin 3 transfected HEK-293T cell lysate; rat colon lysate. ICC/IF: MCF7 cells. IHC-P: HBL435 xenograft tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Galectin 3 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody (ab227249)
Paraffin-embedded HBL435 xenograft tissue stained for Galectin 3 using ab227249 at 1/500 dilution in immunohistochemical analysis.
-
Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Galectin 3 (green) using ab227249 at 1/1000 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
-
All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12% SDS-PAGE gel.
-
All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Galectin 3 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12% SDS PAGE gel-
-
Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution + Rat colon lysate at 50 µg
Predicted band size: 26 kDa12 % SDS-PAGE gel.