Anti-ULBP1 antibody [EPR22088-211] (ab224711)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22088-211] to ULBP1
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ULBP1 antibody [EPR22088-211]
See all ULBP1 primary antibodies -
Description
Rabbit monoclonal [EPR22088-211] to ULBP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: MOLT-4 cells; human peripheral blood mononuclear cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22088-211 -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of Human peripheral blood mononuclear (PBMC) cells labelling ULBP1 with ab224711 at 1/60 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab224711 (Right). Then stained with anti-CD14 conjugated to BV510. Gated on viable cells.
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Flow cytometric analysis of HaCaT (Human skin keratinocyte, Left) / MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Right) cells labelling ULBP1 with ab224711 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: HaCaT. (PMID: 22316211) Gated on viable cells.
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