Anti-SLUG antibody [mAbcam51772] (ab51772)
Key features and details
- Mouse monoclonal [mAbcam51772] to SLUG
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-SLUG antibody [mAbcam51772]
See all SLUG primary antibodies -
Description
Mouse monoclonal [mAbcam51772] to SLUG -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide corresponding to Human SLUG aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab27840) -
Positive control
- Mouse Skin Tissue Lysate
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam51772 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772)
Lane 1 : Untransfected MCF7 cells
Lane 2 : MCF7 cells transfected with SLUG
Lane 3 : MCF7 cells transfected with Snail
Predicted band size: 30 kDa
Observed band size: 30 kDa
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Anti-SLUG antibody [mAbcam51772] (ab51772) at 5 µg/ml +
Mouse skin tissue lysate - total protein (ab4025) at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 34 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes -
All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772) at 1 µg/ml
Lane 1 : Skin tissue lysate from Slug heterozygous mouse
Lane 2 : Skin tissue lysate from Slug knockout mouse
Performed under reducing conditions.
Predicted band size: 30 kDa