Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16998(2)] to Histone H4 (acetyl K20)
Suitable for: ICC/IF, WB
Reacts with: Mouse, Human

Product name

Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)]
See all Histone H4 primary antibodies
Description

Rabbit monoclonal [EPR16998(2)] to Histone H4 (acetyl K20)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysates untreated and treated with 500 ng/ml TSA for 4 hours; NIH/3T3 whole cell lysates untreated and treated with 500 ng/ml TSA for 4 hours. ICC/IF: HeLa and NIH/3T3 cells treated with Trichostatin A (500 ng/ml) for 4 hours.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16998(2)
Isotype

IgG
Research areas

Western blot - Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

All lanes : Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191) at 1/1000 dilution

Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

All lanes : Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191) at 1000 cells

Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with 500 ng/ml TSA for 4 hours

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

All lanes : Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K20ac peptide
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K20 unmodified peptide
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K16Me1 peptide
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K16ac peptide
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml TSA for 4 hours with H4K16 unmodified peptide

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

5 μg peptide is used in each lane of the blocking experiment
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (acetyl K20) with ab177191 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The expression increased on HeLa cells after treatment with Trichostatin A (500 ng/ml) for 4 hours.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab177191 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Histone H4 (acetyl K20) with ab177191 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The expression increased on NIH/3T3 cells after treatment with Trichostatin A (500 ng/ml) for 4 hours.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab177191 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-Histone H4 (acetyl K20) antibody [EPR16998(2)] (ab177191)

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