Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5)
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H2A (acetyl K5) antibody [EP856Y]
See all Histone H2A primary antibodies
Description

Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Rat
IHC-P	Mouse Rat Human
IP	Mouse
WB	Rat

See all applications and species data

Immunogen

Synthetic peptide within Human Histone H2A (N terminal). The exact sequence is proprietary.
Positive control
- Human breast carcinoma tissue and C6 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP856Y
Isotype

IgG
Research areas

Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) This image is courtesy of an anonymous Abreview

Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/1000 dilution (unpurified) + Mouse cortex whole tissue lysate at 20 µg

Secondary
HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 14 kDa
Observed band size: 15 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

ab45152 immunoprecipitating Histone H2A. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1: Mouse brain whole cell lysate
Lane 2: Mouse brain whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45152 in Mouse brain whole cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilution (unpurified)

Lane 1 : C6 cell lysate
Lane 2 : C6 + TSA membrane

Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)

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