Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
Key features and details
- Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl )
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Methylated Lysine (di methyl , mono methyl ) antibody
See all Methylated Lysine primary antibodies -
Description
Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl ) -
Host species
Rabbit -
Specificity
Recognize proteins methylated on lysine residues (mono- & di-methyl lysine). This antibody was affinity purified with mono-methyl lysine and di-methyl lysine, and may also react with tri-methyl lysine. Does not cross-react with acetylated protein. -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Methylated Lysine conjugated to KLH.
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Positive control
- HCL (0.1N) extracted and acetone precipited histone fraction
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6.00
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified using N-methyl (epsilon amino group)lysine on Agarose as affinity matrix. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)Lane 1 : Anti-Methylated Lysine (tri methyl) antibody (does not cross react with mono, or di methyl lysine residues)
Lane 2 : Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
All lanes : Yeast extract
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366) This image is courtesy of an anonymous Abreviewab23366 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed a heat mediated antigen retrieval step was performed in citrate buffer. An HRP conjugated goat anti-rabbit antibody was used as the secondary. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366) This image is courtesy of an anonymous Abreviewab23366 staining formalin fixed paraffin-embedded rat kidney tissue sections. The section was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 5% serum for 30 minutes at 20°C, prior to incubation with the primary antibody (diluted 1/200 in PBS + 5% serum) for 45 mintes at 20°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
