Anti-Histone H4 (mono methyl K20) antibody (ab227947)
Key features and details
- Rabbit polyclonal to Histone H4 (mono methyl K20)
- Suitable for: WB, Dot blot, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (mono methyl K20) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (mono methyl K20) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis
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Immunogen
Synthetic peptide within Human Histone H4 (N terminal) (mono methyl K20). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P62805 -
Positive control
- WB: HeLa whole cell and nuclear lysates. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H4 (mono methyl K20) antibody (ab227947)All lanes : Anti-Histone H4 (mono methyl K20) antibody (ab227947) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 11 kDa15% SDS-PAGE gel.
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Dot Blot - Anti-Histone H4 (mono methyl K20) antibody (ab227947)Dot blot analysis of ab227947 with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227947 at 1/3000 dilution.
Lane 1: Peptide samples of H4K20.
Lane 2: Peptide samples of H4K20me1.
Lane 3: Peptide samples of H4K20me2.
Lane 4: Peptide samples of H4K20me3. -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl K20) antibody (ab227947)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (mono methyl K20) (green) using ab227947 at 1/500 dilution in ICC/IF.
Counter stain: alpha Tubulin, a cytoskeleton marker, stained by an anti-alpha Tubulin antibody at 1/500 dilution (red). Nuclear counterstain: Hoechst 33342 (blue).
Panel A: Histone H4 (methyl K20) protein labeled with ab227947. Panel B: alpha Tubulin labeled with anti-alpha Tubulin antibody. Panel C: Merge of all stainings.
