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Epigenetics and Nuclear Signaling Histones H3 Crotonylated

Anti-Histone H2A (crotonyl K125) antibody [EP18770] (ab195468)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Histone H2A (crotonyl K125) antibody [EP18770] (ab195468)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP18770] to Histone H2A (crotonyl K125)
  • Suitable for: WB, PepArr
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H2A (crotonyl K125) antibody [EP18770]
    See all Histone H2A primary antibodies
  • Description

    Rabbit monoclonal [EP18770] to Histone H2A (crotonyl K125)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, PepArrmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse embryo, testis and ovary lysates; NT2/D1, HL-60, NCCIT, F9, mESC, C2C12, 3T3-L1, NIH/3T3 and HeLa whole cell lysates; human ovary lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP18770
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Crotonylated

Images

  • Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] (ab195468)
    Western blot - Anti-Histone H2A (crotonyl K125) antibody [EP18770] (ab195468)
    All lanes : Anti-Histone H2A (crotonyl K125) antibody [EP18770] (ab195468) at 1/1000 dilution

    Lane 1 : Mouse embryo lysate
    Lane 2 : Mouse testis lysate
    Lane 3 : Human ovary lysate
    Lane 4 : NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 5 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
    Lane 6 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 7 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 8 : Human placenta lysate
    Lane 9 : mESC (Mouse embryonic stem cell line) whole cell lysate
    Lane 10 : Mouse ovary lysate
    Lane 11 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 12 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 13 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 14 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% BSA/TBST.

  • Peptide Array - Anti-Histone H2A (crotonyl K125) antibody [EP18770] (ab195468)
    Peptide Array - Anti-Histone H2A (crotonyl K125) antibody [EP18770] (ab195468)

    ab195468 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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