Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17903] to Histone H4 (crotonyl K5)
Suitable for: ICC/IF, IHC-P, WB, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H4 (crotonyl K5) antibody [EPR17903]
See all Histone H4 primary antibodies
Description

Rabbit monoclonal [EPR17903] to Histone H4 (crotonyl K5)
Host species

Rabbit
Specificity

ab177844 also shows binding to Histone H4 (crotonyl K8) when using high concentrations of a modified peptide (please see the dot-blot image on this datasheet for more information).

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and NIH/3T3 cell lysates. IHC-P: Human stomach, mouse liver and rat pancreas tissues. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17903
Isotype

IgG
Research areas

Western blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

All lanes : Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844) at 1/1000 dilution

Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% BSA /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab177844 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Dot Blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

Dot blot analysis of crotonyl peptide (crotonyl K5) (Lane 1), crotonyl peptide (crotonyl K8) (Lane 2) and non- crotonyl peptide (Lane 3), labeled using ab177844 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human stomach tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat pancreas tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

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