Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21984] to Visfatin - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Visfatin antibody [EPR21984] - BSA and Azide free
    See all Visfatin primary antibodies

  • Description

    Rabbit monoclonal [EPR21984] to Visfatin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bladder cancer tissue.

  • General notes

    Ab240560 is the carrier-free version of ab236873. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240560 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Immunoprecipitation - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

    Visfatin was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma cell line) whole cell lysate with ab236873 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236873 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HTC 116 whole cell lysate 10 µg (Input).
    Lane 2: ab236873 IP in HCT 116 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236873 in HCT 116 whole cell lysate (-).


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236873).

  • Flow Cytometry - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Flow Cytometry - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma cell line) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236873).

  • Flow Cytometry - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Flow Cytometry - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236873).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in rat liver (PMID: 29104634; PMID:24603648) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236873).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in mouse kidney (PMID: 29104634; PMID:24287278) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236873).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human breast cancer(PMID:21784959) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236873).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human bladder cancer (PMID:26396920) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236873).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)
    Anti-Visfatin antibody [EPR21984] - BSA and Azide free (ab240560)

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