Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rabbit polyclonal to Visfatin
Suitable for: IP, WB
Reacts with: Human
Isotype: IgG

Product name

Anti-Visfatin antibody
See all Visfatin primary antibodies
Description

Rabbit polyclonal to Visfatin
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Visfatin aa 450-491 (C terminal). The exact sequence is proprietary.
Database link: P43490
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-Visfatin antibody (ab264146)

All lanes : Anti-Visfatin antibody (ab264146) at 0.04 µg/ml

Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg

Exposure time: 3 seconds

4-20% SDS-PAGE
Immunoprecipitation - Anti-Visfatin antibody (ab264146)

Visfatin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264146 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264146 at 1 µg/ml.

Lane 1: ab264146 IP in HeLa whole cell lysate.
Lane 2: Control IgG.

Exposure time: 3 secs.

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