Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

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Anti-Visfatin antibody [EPR21984] (ab236873)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21984] to Visfatin
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Visfatin antibody [EPR21984]
See all Visfatin primary antibodies
Description

Rabbit monoclonal [EPR21984] to Visfatin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, HT-1080, PC-3, NCI-H460, K562, MCF7, HCT 116, PC-12 and C2C12 whole cell lysates; Human breast cancer, fetal brain, heart and fetal kidney lysates. IHC-P: Human bladder cancer and breast cancer tissues; Mouse kidney tissue; Rat liver tissue. Flow Cyt: K562 and HC T116 cells. IP: HCT 116 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21984
Isotype

IgG
Research areas

Western blot - Anti-Visfatin antibody [EPR21984] (ab236873)

All lanes : Anti-Visfatin antibody [EPR21984] (ab236873) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 4 : C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
Lane 5 : Human fetal brain lysate at 10 µg
Lane 6 : Human heart lysate at 10 µg
Lane 7 : Human fetal kidney lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 55 kDa
Observed band size: 56 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human bladder cancer (PMID:26396920) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Visfatin antibody [EPR21984] (ab236873)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody
Immunoprecipitation - Anti-Visfatin antibody [EPR21984] (ab236873)

Visfatin was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma cell line) whole cell lysate with ab236873 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236873 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: HTC 116 whole cell lysate 10 µg (Input).
Lane 2: ab236873 IP in HCT 116 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236873 in HCT 116 whole cell lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Flow Cytometry - Anti-Visfatin antibody [EPR21984] (ab236873)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma cell line) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in rat liver (PMID: 29104634; PMID:24603648) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in mouse kidney (PMID: 29104634; PMID:24287278) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human breast cancer(PMID:21784959) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Visfatin antibody [EPR21984] (ab236873)

All lanes : Anti-Visfatin antibody [EPR21984] (ab236873) at 1/1000 dilution

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2 : HT1080 (human fibrosarcoma cell line) whole cell lysate at 20 µg
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : NCI-H460 (human lung cancer cell line) whole cell lysate at 20 µg
Lane 5 : Human breast cancer lysate at 20 µg
Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?

Exposure time : Lanes 1-3: 37 seconds; Lanes 4-5: 8 seconds; Lane 6: 26 seconds.

Blocking and diluting buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).
Anti-Visfatin antibody [EPR21984] (ab236873)

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