Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Visfatin antibody [BLR058F] (ab252558)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR058F] to Visfatin
Suitable for: IP, ICC/IF, WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Visfatin antibody [BLR058F]
See all Visfatin primary antibodies
Description

Rabbit monoclonal [BLR058F] to Visfatin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human Visfatin aa 400-450. The exact sequence is proprietary. NP_005737.1
Database link: P43490
Positive control
- IHC-P: Human breast carcinoma and ovarian carcinoma tissue. ICC: NCI-H460. WB: RKO, MCF7, K562, GaMG, HEK-293T, HeLa, U-2 OS, LNCaP, HepG2, Jurkat and NIH/3T3 whole cell lysate. IP: Visfatin IP in HEK-293T whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR058F
Isotype

IgG
Research areas

Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)

All lanes : Anti-Visfatin antibody [BLR058F] (ab252558) at 1/1000 dilution

Lane 1 : RKO (Human cell line) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : GaMG (Human cell line) whole cell lysate
Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 8 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 9 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 10 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 11 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 50 µg per lane.

Predicted band size: 55 kDa

Exposure time: 30 seconds

Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)

Visfatin was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252558 used for IP at 5 µl per reaction. For WB 1/1000 dilution.

Lane 1: ab252558 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293Twhole cell lysate.

Chemiluminescence detection: 75 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558)

FFPE section of NCI-H460 (Human lung cancer cell line) cells labeling Visfatin using ab252558 at a 1/250 dilution in ICC.

Secondary: HRP-conjugated goat anti-rabbit IgG.

Substrate: DAB.
Anti-Visfatin antibody [BLR058F] (ab252558)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com