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Anti-Visfatin antibody [BLR058F] (ab252558)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR058F] to Visfatin
  • Suitable for: IP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Visfatin antibody [BLR058F]
    See all Visfatin primary antibodies

  • Description

    Rabbit monoclonal [BLR058F] to Visfatin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Visfatin aa 400-450. The exact sequence is proprietary. NP_005737.1
    Database link: P43490

  • Positive control

    • IHC-P: Human breast carcinoma and ovarian carcinoma tissue. ICC: NCI-H460. WB: RKO, MCF7, K562, GaMG, HEK-293T, HeLa, U-2 OS, LNCaP, HepG2, Jurkat and NIH/3T3 whole cell lysate. IP: Visfatin IP in HEK-293T whole cell lysate.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Images

  • Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)
    Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)
    All lanes : Anti-Visfatin antibody [BLR058F] (ab252558) at 1/1000 dilution

    Lane 1 : RKO (Human cell line) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : GaMG (Human cell line) whole cell lysate
    Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 7 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 8 : LNCaP (Human prostate cancer cell line) whole cell lysate
    Lane 9 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 10 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 11 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 55 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)
    Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)

    Visfatin was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab252558 used for IP at 5 µl per reaction. For WB 1/1000 dilution.

    Lane 1: ab252558 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293Twhole cell lysate.

    Chemiluminescence detection: 75 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)

    Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

  • Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558)
    Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558)

    FFPE section of NCI-H460 (Human lung cancer cell line) cells labeling Visfatin using ab252558 at a 1/250 dilution in ICC.

    Secondary: HRP-conjugated goat anti-rabbit IgG.

    Substrate: DAB.

  • Anti-Visfatin antibody [BLR058F] (ab252558)
    Anti-Visfatin antibody [BLR058F] (ab252558)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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