Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR058F] to Visfatin - BSA free
  • Suitable for: IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Visfatin antibody [BLR058F] - BSA free
    See all Visfatin primary antibodies

  • Description

    Rabbit monoclonal [BLR058F] to Visfatin - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Visfatin aa 400-450. The exact sequence is proprietary. NP_005737.1
    Database link: P43490

  • Positive control

    • IHC-P: Human breast carcinoma and ovarian carcinoma tissue. ICC: NCI-H460. IP: Visfatin IP in HEK-293T whole cell lysate.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272084 is the carrier-free version of ab252558. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Properties

Images

  • Immunoprecipitation - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)
    Immunoprecipitation - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)

    Visfatin was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab252558 used for IP at 5 µl per reaction. For WB 1/1000 dilution.

    Lane 1: ab252558 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293Twhole cell lysate.

    Chemiluminescence detection: 75 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252558).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)

    Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252558).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252558).

  • Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)
    Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)

    FFPE section of NCI-H460 (Human lung cancer cell line) cells labeling Visfatin using ab252558 at a 1/250 dilution in ICC.

    Secondary: HRP-conjugated goat anti-rabbit IgG.

    Substrate: DAB.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252558).

  • Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)
    Anti-Visfatin antibody [BLR058F] - BSA free (ab272084)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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