Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dux4 antibody [E5-5] (ab124699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E5-5] to Dux4
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Dux4 antibody [E5-5]
    See all Dux4 primary antibodies

  • Description

    Rabbit monoclonal [E5-5] to Dux4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment aa 300-450. The exact sequence is proprietary.

  • Positive control

    • WB: Human testis tissue lysates. HT-1080, PC-3 Saos-2 and HeLa whole cell lysate (ab150035).

  • General notes

    For details on the development of this clone, please refer to Geng et al.  Hybridoma (Larchmt). 2011 Apr; 30(2): 125–130.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    E5-5

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Dux4 antibody [E5-5] (ab124699)
    Western blot - Anti-Dux4 antibody [E5-5] (ab124699)
    All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

    Lane 1 : HT1080 (human fibrosarcoma cell line) whole cell lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : Saos-2 (human osteosarcoma cell line) whole cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dux4 antibody [E5-5] (ab124699)
    Western blot - Anti-Dux4 antibody [E5-5] (ab124699)
    All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)

    Lane 1 : Human testis lysate
    Lane 2 : HT1080 (human fibrosarcoma cell line) cell lysate
    Lane 3 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 45 kDa

  • Anti-Dux4 antibody [E5-5] (ab124699)
    Anti-Dux4 antibody [E5-5] (ab124699)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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