Mouse OB Cadherin ELISA Kit (ab208346)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse OB Cadherin ELISA Kit
See all OB Cadherin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 5 3.5% Inter-assay Sample n Mean SD CV% serum 3 14.2% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
7 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 111 109% - 115% Cell culture media 87 76% - 94% Hep Plasma 116 112% - 122% EDTA Plasma 114 106% - 125% Cit plasma 103 101% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow, Pig -
Product overview
Mouse OB Cadherin ELISA Kit (ab208346) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OB Cadherin protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, urine, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse OB Cadherin with 7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
OB Cadherin (also known as Cadherin-11) is a member of the Cadherin family of calcium-dependent cell adhesion proteins. OB Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse OB Cadherin Capture Antibody 1 x 600µl 10X Mouse OB Cadherin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse OB Cadherin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts. -
Involvement in disease
Note=A chromosomal aberration involving CDH11 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with USP6. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CAD11
- CAD11_HUMAN
- Cadherin 11
see all -
Database links
- Entrez Gene: 12552 Mouse
- SwissProt: P55288 Mouse
- Unigene: 1571 Mouse
Images
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Other - Mouse OB Cadherin ELISA Kit (ab208346)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse OB Cadherin standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native OB-Cadherin in mouse serum, plasma (citrate) and plasma (EDTA) samplesThe concentrations of OB Cadherin were measured in duplicates, interpolated from the OB Cadherin standard curves and corrected for sample dilution. 1X diluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OB Cadherin concentration was determined to be 3,771 pg/mL in serum, 827 pg/mL in plasma (citrate) and 1,172 pg/mL in plasma (EDTA).
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Interpolated concentrations of native OB-Cadherin in mouse plasma (heparin) and urine samplesThe concentrations of OB Cadherin were measured in duplicates, interpolated from the OB Cadherin standard curves and corrected for sample dilution. 1X diluted samples are as follows: plasma (heparin) 25%, and urine 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OB Cadherin concentration was determined to be 1,343 pg/mL in plasma (heparin) and 1,000 pg/mL in urine.
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Sandwich ELISA - Mouse OB Cadherin ELISA Kit (ab208346)To learn more about the advantages of recombinant antibodies see here.
