Mouse OB Cadherin Matched Antibody Pair Kit (ab216066)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.25 pg/ml - 2000 pg/ml
Overview
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Product name
Mouse OB Cadherin Matched Antibody Pair Kit
See all OB Cadherin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
17.2 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse OB Cadherin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse OB Cadherin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208346. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse OB Cadherin Capture Antibody 2 x 50µg 1 x 50µg Mouse OB Cadherin Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse OB Cadherin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts. -
Involvement in disease
Note=A chromosomal aberration involving CDH11 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with USP6. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CAD11
- CAD11_HUMAN
- Cadherin 11
see all -
Database links
- Entrez Gene: 12552 Mouse
- SwissProt: P55288 Mouse
- Unigene: 1571 Mouse
Images
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Mouse OB Cadherin standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse OB Cadherin Matched Antibody Pair Kit (ab216066)To learn more about the advantages of recombinant antibodies see here.
