Anti-Nkx2.2 antibody (ab272915)
Key features and details
- Rabbit polyclonal to Nkx2.2
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nkx2.2 antibody
See all Nkx2.2 primary antibodies -
Description
Rabbit polyclonal to Nkx2.2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Mouse Nkx2.2 (C terminal). The exact sequence is proprietary.
Database link: P42586 -
Positive control
- IHC-Fr: Rat E13.5 brain tissue. ICC/IF: DIV9 rat E18 primary hippocampal neuron cells. WB: HEK-293T cells transfected with DDDDK-tagged Nkx2.2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Nkx2.2 antibody (ab272915)
Paraffin-embedded rat E13.5 brain tissue stained for Nkx2.2 using ab272915 at 1/250 dilution in immunohistochemical analysis.
Red: Beta tubulin.
Blue: DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody (ab272915)4% PFA-fixed DIV9 rat E18 primary hippocampal neuron cells stained for Nkx2.2 using ab272915 at 1/500 dilution in ICC/IF.
Red: Beta tubulin.
Blue: DAPI.
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Western blot - Anti-Nkx2.2 antibody (ab272915)All lanes : Anti-Nkx2.2 antibody (ab272915) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T cell lysate
Lane 2 : DDDDK-tagged Nkx2.2 transfected HEK-293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE.