Anti-Chk1 (phospho S286) antibody (ab62243)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S286)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 (phospho S286) antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 (phospho S286) -
Host species
Rabbit -
Specificity
ab62243 detects endogenous levels of Chk1 only when phosphorylated at serine 286. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 286 (S-E-SP-P-S).
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Positive control
- HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab62243 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Chk1 (phospho S286) antibody (ab62243)ab62243 at 1/500 - 1/1000 dilution staining Chk1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
