L-Lactate Assay Kit (Colorimetric) (ab65331)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 0.02 mM
Overview
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Product name
L-Lactate Assay Kit (Colorimetric)
See all L-Lactate kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
0.02 mM -
Range
0.02 mM - 10 mM -
Assay time
0h 30m -
Product overview
L-Lactate Assay Kit (Colorimetric) (ab65331) uses an assay protocol where lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm).
The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, tissues, cell culture and fermentation media.
Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
Notes
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see D-Lactate assay kits) but only at about 1-5% of the concentration of L(+)-Lactate.
L-Lactate assay kit ab65331 is our most popular L-Lactate assay kit (colorimetric 450nm, range 0.02 mM - 10 mM). Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
- L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM
- L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.How other researchers have used L-Lactate Assay Kit ab65331
This Lactate assay kit has been used in publications in a variety of sample types, including:
- Human: THP-1 cell lysates1, MDA-MB-231 and HepG2 cell culture lysates2, cell culture supernatant (HepG2, A549, Huh7, PC3, LN229, HeLa)3, brain tissue4
- Mouse: brown adipose tissue lysate5, thymic lymphoma tissue6, cell culture supernatant7, T cell primary cell culture supernatants8, serum9, serum and muscle10
- Bovine: cumulus cell culture supernatant11References: 1 - Tran UT and Kitami T 2019; 2 - Cui J et al 2019; 3 - Rodriguez ML et al 2018, Chen Y et al 2018, Zhang D et al 2018, Caino MC et al 2017, Birkenmeier K et al 2015; 4 - Sullivan RC et al 2019; 5 - Jeong JH et al 2018; 6 - Vara-Ciruelos D et al 2019; 7 - Fiorenzano et al 2016; 8 - Menk AV et al 2018; 9 - Deng W et al 2019, Guglielmetti C et al 2017, Kang R et al 2016; 10 - Kim HY et al 2016; 11 - Sinha et al 2017
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl Lactate Assay Buffer WM 1 x 25ml Lactate Enzyme Mix (lyophilized) Green 1 vial Lactate Substrate Mix Red 1 vial -
Research areas
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Relevance
Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).
Images
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L-Lactate Assay Kit (Colorimetric) ab65331 Image from Kelly CE et al., PLoS Biol 11(4), fig2d. oi: 10.1371/journal.pbio.1001538. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Plasma lactate concentrations were determined using L-Lactate assay kit (ab65331) in Ark2C+/+ and Ark2C−/− (Arkadia-like gene) mice.
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ab65331 L-Lactate Assay Kit (Colorimetric)Linearity of dilution: concentration of L-Lactate in differently diluted (X-axis) biological samples, demonstrating a linearity of 89%-111% (concentrations corrected for by factor of dilution; duplicates; +/- SD).
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L-Lactate Assay Kit (Colorimetric) (ab65331)Relative signal (RFU) in unfiltered human plasma (dilution 1:8), comparing L-lactate signals with background reading (no enzyme) after 10 minutes of incubation (duplicates +/- SD).
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ab65331 L-Lactate Assay Kit (Colorimetric)Standard curve with background signal subtracted (duplicates; +/- SD).
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L-Lactate Assay Kit (Colorimetric) (ab65331)Lactate Standard Curve. The assay is performed following the kit (ab65331) protocol.
