L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see D-Lactate assay kits) but only at about 1-5% of the concentration of L(+)-Lactate.

L-Lactate assay kit ab65331 is our most popular L-Lactate assay kit (colorimetric 450nm, range 0.02 mM - 10 mM). Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
- L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM
- L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM

Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

How other researchers have used L-Lactate Assay Kit ab65331

This Lactate assay kit has been used in publications in a variety of sample types, including:
- Human: THP-1 cell lysates¹, MDA-MB-231 and HepG2 cell culture lysates², cell culture supernatant (HepG2, A549, Huh7, PC3, LN229, HeLa)³, brain tissue⁴
- Mouse: brown adipose tissue lysate⁵, thymic lymphoma tissue⁶, cell culture supernatant⁷, T cell primary cell culture supernatants⁸, serum⁹, serum and muscle¹⁰
- Bovine: cumulus cell culture supernatant¹¹ 

^{References: 1 - Tran UT and Kitami T 2019; 2 - Cui J et al 2019; 3 - Rodriguez ML et al 2018, Chen Y et al 2018, Zhang D et al 2018, Caino MC et al 2017, Birkenmeier K et al 2015; 4 - Sullivan RC et al 2019; 5 - Jeong JH et al 2018; 6 - Vara-Ciruelos D et al 2019; 7 - Fiorenzano et al 2016; 8 - Menk AV et al 2018; 9 - Deng W et al 2019, Guglielmetti C et al 2017, Kang R et al 2016; 10 - Kim HY et al 2016; 11 - Sinha et al 2017}

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