Anti-DR4 antibody (ab216662)
Key features and details
- Rabbit polyclonal to DR4
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DR4 antibody
See all DR4 primary antibodies -
Description
Rabbit polyclonal to DR4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide aa 420-468 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O00220 -
Positive control
- IHC-P; Rat uterus and colon tissue. Human colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-DR4 antibody (ab216662) at 1/500 dilution + MCF-7 cell lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat uterus tissue labeling DR4 with ab216662 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling DR4 with ab216662 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling DR4 with ab216662 at 1/500 dilution overnight at 4°C followed by conjugation to the secondary antibody and DAB staining. Prior to addition of the primary antibody, antigen retrieval was carried out by boiling in sodium citrate buffer (pH6.0) for 15min. Endogenous peroxidase was blocked by 3% hydrogen peroxide for 30 minutes and blocking buffer (normal goat serum) added at 37°C for 20 min.