Human Granulysin ELISA Kit (ab256402)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.22 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Granulysin ELISA Kit
See all GNLY/Granulysin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.5% Inter-assay Sample n Mean SD CV% Serum 3 0.4% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.22 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 117 114% - 119% Serum 100 98% - 102% EDTA Plasma 112 103% - 120% Cit plasma 104 102% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Granulysin ELISA Kit (ab256402) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Granulysin protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Granulysin with 8.22 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Granulysin (GNLY) is a 15.5 kD protein is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is a microbiocidal via induction of apoptosis. The gene is expressed in cytolytic granules with perforin, a pore forming protein as well as granzymes that are also involved in cytolysis. In addition to it. As an antimicrobial, Granulysin’s activities include killing microbes that cause tuberculosis and malaria. It has been reported to be involved in tumor destruction. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. Granulysin shares 76% and 26% sequence homology with monkey and mouse, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Granulysin Capture Antibody 1 x 600µl 10X Human Granulysin Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Granulysin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. -
Tissue specificity
Expressed in natural killer and T-cells. -
Sequence similarities
Contains 1 saposin B-type domain. -
Post-translational
modificationsA 9 kDa form is produced by proteolytic processing of a 15 kDa protein. -
Cellular localization
Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation. - Information by UniProt
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Alternative names
- 519
- D2S69E
- GNLY
see all -
Database links
- Entrez Gene: 10578 Human
- Omim: 188855 Human
- SwissProt: P22749 Human
- Unigene: 105806 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Granulysin standard curve in Sample Diluent NS.The Granulysin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Granulysin in human serum, plasma and cell culture supernatant samples.The concentrations of Granulysin were measured in duplicates, interpolated from the Granulysin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and stimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 1153.29 pg/mL in serum, 790.67 pg/mL in plasma (citrate), 1668.29 pg/mL in plasma (EDTA), and 3387.74 pg/mL in stimulated PBMC supernatant.
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Interpolated concentrations of native Granulysin in NK-92 cell culture supernatant samples.The concentrations of Granulysin were measured in duplicates, interpolated from the Granulysin standard curves and corrected for sample dilution. Undiluted samples are as follows: NK-92 cell culture supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 130.06 ng/mL in NK-92 supernatant.
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Interpolated concentrations of native Granulysin in human placenta extract based on a 250 µg/mL extract load.The concentrations of Granulysin were measured in duplicate and interpolated from the Granulysin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 5888.16 pg/mL in placenta extract.
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Neat unstimulated and PHA-M stimulated PBMC cell culture were measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 274.19 pg/mL in unstimulated PBMC supernatant 3720.93 pg/mL in stimulated PBMC supernatant.
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50% Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 2536.17 pg/mL with a range of 500.32 – 5863.75 pg/mL.
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Sandwich ELISA - Human Granulysin ELISA Kit (ab256402)To learn more about the advantages of recombinant antibodies see here.
