Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCIF1 antibody [EPR24319-21] (ab271081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24319-21] to PCIF1
  • Suitable for: IP, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PCIF1 antibody [EPR24319-21]
    See all PCIF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24319-21] to PCIF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, MCF-7, HeLa, Jurkat, Human testis, Rat liver, Rat testis lysates. IP: MCF-7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24319-21

  • Isotype

    IgG

Images

  • Western blot - Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    Western blot - Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    All lanes : Anti-PCIF1 antibody [EPR24319-21] (ab271081) at 1/1000 dilution

    Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 81 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot (lane 3 and 4) was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Western blot - Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    Western blot - Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    Anti-PCIF1 antibody [EPR24319-21] (ab271081) at 1/1000 dilution + Human testis tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 81 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

     Exposure time: 3 minutes.

     

  • Western blot - Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    Western blot - Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    All lanes : Anti-PCIF1 antibody [EPR24319-21] (ab271081) at 1/1000 dilution

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 81 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    Immunoprecipitation - Anti-PCIF1 antibody [EPR24319-21] (ab271081)

    PCIF1 was immunoprecipitated from 0.35 mg MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab271081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271081 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab271081 IP in MCF-7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271081 in MCF-7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

    This blot was developed using a higher sensitivity ECL substrate.

  • Anti-PCIF1 antibody [EPR24319-21] (ab271081)
    Anti-PCIF1 antibody [EPR24319-21] (ab271081)

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