Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15373(B)] to ABCE1 - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free
    See all ABCE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15373(B)] to ABCE1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250835 is the carrier-free version of ab185548. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Western blot - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    All lanes : Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    This data was developed using ab185548, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

    This data was developed using ab185548, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    This data was developed using ab185548, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded mouse liver tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    This data was developed using ab185548, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling ABCE1 using ab185548 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.
  • Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Immunocytochemistry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    This data was developed using ab185548, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of formaldehyde fixed 293 cells labeling ABCE1 using ab185548 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.
  • Flow Cytometry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Flow Cytometry - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    This data was developed using ab185548, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of K562 cells labeling ABCE1 using ab185548 at a 1/220 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
  • Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    This data was developed using ab185548, the same antibody clone in a different buffer formulation.Western blot analysis of ABCE1 in immunoprecipitation pellets from 293 lysate (Lane 1) or 1XPBS (negative control) (Lane 2) using ab185548 at 1/50 dilution. Secondary antibody, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.
  • Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)
    Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free (ab250835)

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