Human PGRPs ELISA Kit (ab267571)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 6 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PGRPs ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6 pg/ml -
Range
6 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 114 99% - 128% Plasma 107.7 91% - 119% Cell culture media 103.7 83% - 123% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human PGRPs ELISA Kit is designed for the quantitative determination of PGRPs in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human PGRPs coated on a 96-well plate. Standards and samples are pipetted into the wells and PGRPs present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PGRPs antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGRPs bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA antibody pair detects human PGRPs, less than 0.05% cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human PGRPs coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human PGRPs Detection Antibody 2 vials Human PGRPs Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity. -
Tissue specificity
Highly expressed in bone marrow. Weak expression found in kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lung, fetal spleen and neutrophils. -
Sequence similarities
Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. -
Post-translational
modificationsN-glycosylated. N-glycosylation is required for bactericidal activity. -
Cellular localization
Secreted. Cytoplasmic granule. - Information by UniProt
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Alternative names
- MGC126894
- MGC126896
- Peptidoglycan recognition protein
see all -
Database links
- Entrez Gene: 8993 Human
- Omim: 604963 Human
- SwissProt: O75594 Human
- Unigene: 137583 Human
Images
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Human PGRPs ELISA kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.