Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDE7B antibody [EPR11491] (ab170914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11491] to PDE7B
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PDE7B antibody [EPR11491]
    See all PDE7B primary antibodies

  • Description

    Rabbit monoclonal [EPR11491] to PDE7B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PDE7B aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NP56

  • Positive control

    • MCF-7; HeLa; HepG2 and BxPC-3 cell lysates; Human fetal brain tissue lysate; HeLa cells; Human brain tissue; Human heart tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PDE7B antibody [EPR11491] (ab170914)
    Immunocytochemistry/ Immunofluorescence - Anti-PDE7B antibody [EPR11491] (ab170914)

    Immunofluorescent analysis of HeLa cells labeling PDE7B using ab170914 at 1/500 dilution.  DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (ab170914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (ab170914)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling PDE7B with ab170914 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (ab170914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7B antibody [EPR11491] (ab170914)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PDE7B with ab170914 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-PDE7B antibody [EPR11491] (ab170914)
    Flow Cytometry - Anti-PDE7B antibody [EPR11491] (ab170914)

    Flow cytometric analysis of permeabilized Hela cells labeling PDE7B using ab170914 at 1/500 dilution  (red) or a rabbit IgG negative (green).

  • Western blot - Anti-PDE7B antibody [EPR11491] (ab170914)
    Western blot - Anti-PDE7B antibody [EPR11491] (ab170914)
    All lanes : Anti-PDE7B antibody [EPR11491] (ab170914) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : BxPC-3 cell lysate
    Lane 5 : Human fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 52 kDa

  • Anti-PDE7B antibody [EPR11491] (ab170914)
    Anti-PDE7B antibody [EPR11491] (ab170914)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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