Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)
Key features and details
- Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
See all IgG secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Rabbit IgG, whole molecule
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Conjugation
Cy5 ®. Ex: 650nm, Em: 667nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
Conjugation notes
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Images
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Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564) Hennenberg, M. et al PLoS One. 2012;7(11):e50904. doi: 10.1371/journal.pone.0050904. Epub 2012 Nov 30 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunofluorescence stainings of human prostate tissues
Sections were double labeled for different PAK isoforms, together with calponin (marker for smooth muscle cells), pan-cytokeratin (marker for epithelial cells of glands), or tyrosine hydroxylase (TH, marker for catecholaminergic nerves). Yellow color in merged pictures may indicate colocalization of targets. Shown are representative stainings from series with tissues from n = 5 patients for each combination.
ab6908 was used as secondary antibody to visualize various rabbit anti-PAK primary antibodies (red).
(From Figure 2 of Hennenberg et al)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564) This image is courtesy of an anonymous Abreview.ab11175 staining Histone H2A.X in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 4% BSA for 2 hours at 37°C. Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS/4% BSA/0.05% Triton X-100) for 48 hours at 4°C. A Cy5®-conjugated goat anti-rabbit IgG H&L preadsorbed (ab6564) (1/300) was used as the secondary antibody.
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Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564) -
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)
