Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)
![Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)](https://www.abcam.com/ps/products/193/ab193185/Images/ab193185-230575-anti-human-ig-light-chain-antibody-rm129-biotin-sandwich-elisa.jpg "Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM129] to Human Ig light chain
- Suitable for: Sandwich ELISA, ELISA
- Reacts with: Human, Recombinant fragment
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-Human Ig light chain antibody [RM129]
See all Human Ig light chain primary antibodies -
Description
Biotin Rabbit monoclonal [RM129] to Human Ig light chain -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragment
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Immunogen
Full length protein corresponding to Human IgG. Purified Native Human IgG from Human Plasma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48.91% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A affinity purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM129 -
Isotype
IgG -
Research areas
Images
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Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)
A titer Sandwich ELISA using ab193185 as the detection antibody. The plate, coated with the capture antibody anti-human IgG, was loaded with different amounts of human IgG. A serial dilution of ab193185 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.
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![ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)](https://www.abcam.com/ps/products/193/ab193185/Images/ab193185-230574-anti-human-ig-light-chain-antibody-rm129-biotin-elisa.jpg)
ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)ELISA of human immunoglobulins shows ab193185 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50ng/well of different immunglobulins. 100 ng/mL or 20 ng/mL was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)](https://www.abcam.com/ps/products/193/ab193185/Images/ab193185-3-benefits-of-recombinant-antibodies.png)
Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)