Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4119] to MCM2 (phospho S27)
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-MCM2 (phospho S27) antibody [EPR4119]
See all MCM2 primary antibodies
Description

Rabbit monoclonal [EPR4119] to MCM2 (phospho S27)
Host species

Rabbit
Specificity

This antibody only detects MCM2 when phosphorylated at serine 27.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

A phospho specific peptide corresponding to residues surrounding serine 27 of Human MCM2.
Positive control
- HeLa whole cell lysate (ab150035) and HeLa cells. Human cervical carcinoma tissue. Human tonsil tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4119
Isotype

IgG
Research areas

Western blot - Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459) at 1/10000 dilution

Lane 1 : HeLa cell lysates untreated with alkaline phosphatase
Lane 2 : HeLa cell lysates treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

MCM2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) with ab109459 at 1/100 dilution.

Exposue time:10 second.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

ab109459 at 1/100 dilution staining MCM2 (phospho S27) in HeLa cells by Immunofluorescence.
Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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