Anti-MCM2 (phospho S108) antibody (ab264114)
Key features and details
- Rabbit polyclonal to MCM2 (phospho S108)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MCM2 (phospho S108) antibody
See all MCM2 primary antibodies -
Description
Rabbit polyclonal to MCM2 (phospho S108) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human MCM2 (phospho S108). The exact sequence is proprietary.
Database link: P49736 -
Positive control
- IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissue. WB: HEK-293T and NIH/3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM2 (phospho S108) antibody (ab264114)All lanes : Anti-MCM2 (phospho S108) antibody (ab264114) at 1/5000 dilution
Lane 1 : HEK-293T treated with etoposide (100 µM, 2h), then following immunoprecipitation, treated with phosphatases
Lane 2 : HEK-293T treated with etoposide (100 µM, 2h), then following immunoprecipitation, mock treated with phosphatases
Lane 3 : HEK-293T mock treated with etoposide (100 µM, 2h), then following immunoprecipitation, treated with phosphatases
Lane 4 : HEK-293T mock treated with etoposide (100 µM, 2h), then following immunoprecipitation, mock treated with phosphatases
Lane 5 : HEK-293T treated with etoposide (100 µM, 2h), then following immunoprecipitation, with normal IgG, treated with phosphatases
Predicted band size: 102 kDa
Exposure time: 10 secondsAn alternative anti-phospho MCM2 (S108) antibody was used for the immunoprecipitation. ab264114 was used for Western Blotting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S108) antibody (ab264114)Formalin/PFA-fixed paraffin-embedded sections of human breast carcinoma (left panel) and mouse renal cell carcinoma (right panel) stained for MCM2 using ab264114 at a 1/5000 dilution. Detection: DAB.
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Western blot - Anti-MCM2 (phospho S108) antibody (ab264114)All lanes : Anti-MCM2 (phospho S108) antibody (ab264114) at 1/5000 dilution
Lane 1 : HEK-293T whole cell lysate treated with 100µM etoposide for 2 hours (+) at 50 µg
Lane 2 : HEK-293T whole cell lysate mock treated (-) at 50 µg
Lane 3 : HEK-293T whole cell lysate treated with 100µM etoposide for 2 hours (+) at 15 µg
Lane 4 : HEK-293T whole cell lysate mock treated (+) at 15 µg
Lane 5 : NIH/3T3 whole cell lysate treated with 100µM etoposide for 2 hours (+) at 50 µg
Predicted band size: 102 kDaDetection: Chemiluminescence with an exposure time of 10 seconds
