Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP4120] to MCM2 (phospho S53)
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-MCM2 (phospho S53) antibody [EP4120]
See all MCM2 primary antibodies
Description

Rabbit monoclonal [EP4120] to MCM2 (phospho S53)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysate (ab150035). IHC-P:Human tonsil tissue. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP4120
Isotype

IgG
Research areas

Western blot - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

All lanes : Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133) at 1/10000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates and the membrane was incubated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ab109133 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Immunofluorescent staining of HeLa cells using ab109133 at a dilution of 1/50.
Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com