Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HCLS1 antibody [EPR16540] (ab202900)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16540] to HCLS1
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HCLS1 antibody [EPR16540]
    See all HCLS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16540] to HCLS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos and Daudi cell lysates. IHC-P: Human tonsil tissue; Human Kupffer cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16540

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HCLS1 antibody [EPR16540] (ab202900)
    Western blot - Anti-HCLS1 antibody [EPR16540] (ab202900)
    All lanes : Anti-HCLS1 antibody [EPR16540] (ab202900) at 1/5000 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
    Lane 2 : Daudi (Human Burkitt's lymphoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 54 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] (ab202900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] (ab202900)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HCLS1 with ab202900 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and weakly nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] (ab202900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] (ab202900)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HCLS1 with ab202900 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on Kupffer cells, while no staining on Human liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] (ab202900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] (ab202900)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HCLS1 with ab202900 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-HCLS1 antibody [EPR16540] (ab202900)
    Anti-HCLS1 antibody [EPR16540] (ab202900)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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